Flexible control

At Lightcast we are developing a platform that combines the flexibility to analyse a wide range of cell types and assays with precise control over droplet number, occupancy, location and movement. Together this enables efficient, multi-step workflows that scale to match your experimental needs.
Technology

Redefine your workflow

Lightcast provides a flexible platform for precisely interrogating single-cell interactions and functional behaviours at scale.

Precise interrogation of single cell interactions and functional behaviours at scale

Precise interrogation of single cell interactions

and functional behaviours at scale

Load

Droplet generation & loading

  • 4 specific inputs

  • Rapidly generate millions of droplets

  • Optimised for fragile samples

Filter

Droplet acquisition & filtering

  • Acquisition by light sprites provides precise experimental control

  • Filter droplets for size and content

  • Filter criteria automatically defines array formation strategy

Array

Droplet arraying

  • Machine-learning-optimised array

  • “Target” droplets contstruct the array while “Disqualified” droplets are discarded

  • Array is environmentally controlled

Merge

Sample merging

  • Merge samples by FOV or across the entire chipc inputs

  • Massively parallel merging of interdigitated sample droplets

  • Conduct complex experiments with multiple sequential merges

Assay

Sample assaying

  • Define brightfield and fluorescence imaging according to your experimental needs

  • Identify and select hits with a broad range of functional reporters

  • Process in real time for down-selection, sequential processing, or dispensing

Identify antigen-binding antibody secretor cells using dynamic single cell analysis
Dispense

Sample dispensing

  • Dispense selected hits into 96-well plates in real time

  • Single or pooled cell dispensing based on experimental design

  • Trace each droplet digitally, from load to dispense.

Precise interrogation of single cell interactions and functional behaviours at scale

Load

Droplet generation & loading

  • 8 specific inputs

  • Rapidly generate millions of droplets

  • Optimised for fragile samples

Filter

Droplet acquisition & filtering

  • Acquisition by light sprites provides precise experimental control

  • Filter droplets for size and content

  • Filter criteria automatically defines array formation strategy

Array

Droplet arraying

  • Machine-learning-optimised array

  • “Target” droplets contstruct the array while “Disqualified” droplets are discarded

  • Array is environmentally controlled

Merge

Sample merging

  • Merge samples by FOV or across the entire chipc inputs

  • Massively parallel merging of interdigitated sample droplets

  • Conduct complex experiments with multiple sequential merges

Assay

Sample assaying

Identify antigen-binding antibody secretor cells using dynamic single cell analysis
  • Define brightfield and fluorescence imaging according to your experimental needs

  • Identify and select hits with a broad range of functional reporters

  • Process in real time for down-selection, sequential processing, or dispensing

Dispense

Sample dispensing

  • Dispense selected hits into 96-well plates in real time

  • Single or pooled cell dispensing based on experimental design

  • Trace each droplet digitally, from load to dispense.

Advancing drug development with function-focused profiling at the single-cell level

Download and read the latest Lightcast white paper.

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